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. 1999 Jun 22;96(13):7433–7438. doi: 10.1073/pnas.96.13.7433

Figure 4.

Figure 4

Replication of CTV hybrids containing 5′ and/or 3′ terminal exchanges. Shown is the accumulation of positive- (upper panels) and negative-stranded (lower panels) RNAs of CTV in protoplasts at 2 and 3 dpi with transcripts from pCTV-ΔCla (T36) and pCTV-ΔCla hybrids containing 5′ NTR sequences of VT, T3, T68, and T30 isolates (A), 5′ 1-kb sequences of VT and T30 (B), pCTV-ΔCla-3′NTR (T36) and pCTV-ΔCla-3′NTR hybrids containing heterologous 3′ NTRs (C), and both 5′ and 3′ NTRs of VT and T30 (D). The blots were exposed for 13 min except, T36 5′ NTR, which was exposed for 6 min. The position of genomic and sgRNAs in each panel are indicated by arrow and arrow heads, respectively.