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Apparent molecular weight (MWapp) of class II MHC/ligand complexes loaded with monomeric or dimeric ligand constructs. (A) Soluble HLA-DR1 or -DR4 molecules loaded with HA306-318 peptide (P lanes) or dimeric ligand constructs consisting of two HA306-318 epitope units linked by a tetraethylene glycol spacer (D lanes) were separated by SDS/PAGE. The MWapp indicated in the figure refers to bands representing distinct MHC/ligand complexes. Gels were visualized by silver staining.
