Figure 5.

Determination of the apparent size of F- and C-forms of HLA-DR1/ligand complexes by size exclusion chromatography. The elution profiles of HLA-DR1/ligand complexes formed with ligands containing the HA306-318 (Upper) and IC106-120 epitope (Lower) are shown. In the left panels, the elution of peptide-loaded C-forms is compared with the elution of the F-forms generated with the teg-linked YD hybrid dimers (indicated in the figure). As a reference, elution volumes of marker proteins and their molecular weights are indicated. The right panels show a comparison of the elution of the ligand-loaded forms (solid lines) to the elution of empty HLA-DR1 preparations (dashed lines).