Figure 1.

Two-color flow cytometric analysis and cytolytic activity of IEL isolated from wt, β^−/− and α^−/− mice at 2–3 months of age. (Upper) Representative staining of αβ- and γδ-IEL. IEL were incubated first with anti-TCR-αβ mAb (biotinylated) and then with streptavidin–phycoerythrin (Caltag, South San Francisco, CA) and anti-TCR-γδ mAb (FITC-conjugated). Percentage of positive cells in the corresponding quadrants is shown. (Lower) The redirected lysis assay was done in the presence of anti-TCR-αβ mAb (○) or anti-TCR-γδ mAb (●). The results are means ± SD of data obtained from three independent analyses of five mice per group.