Figure 3.

Two-color flow cytometric analysis and cytolytic activity of IEL isolated from wt, δ^−/−, and IL-7R^−/− mice at 2–3 months of age. (A) Representative staining of αβ- and γδ-IEL. IEL were incubated first with anti-TCR-αβ mAb (biotinylated) and then with streptavidin–phycoerythrin and anti-TCR-γδ mAb (FITC-conjugated). Percentage of positive cells in the corresponding quadrants is shown. (B) Redirected cytolytic activity of αβ-IEL from wt (○), δ^−/− (●), and IL-7R^−/− (□) mice in the presence of anti-TCR-αβ mAb. The results are means ± SD of data obtained from three independent analyses of four to five mice per group.