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IFN-γ production by IEL isolated from wt (n = 14), β−/− (n = 9) and α−/− (n = 9) mice at 2–3 months of age. Two × 105 IEL were cultured in either uncoated, ant-CD3 mAb-coated, or anti-TCR-γδ mAb-coated culture plates. Supernatants were collected on day 3, and the concentration of IFN-γ in the corresponding supernatants was measured by ELISA.
