Table 1.

γδ-IEL isolated from various mouse strains

Mice n	γδ-IEL per mouse, × 106	Subset of γδ-IEL, %
		Thy-1+	Vγ1+	Vγ4+	Vγ7+	Vδ4+
wt 8	3.05 ± 0.68	27.5 ± 10.9	34.7 ± 5.7	10.3 ± 4.1	52.8 ± 11.8	14.0 ± 3.6
β−/− 9	4.24 ± 1.06	23.1 ± 1.6	55.3 ± 4.5	8.2 ± 1.4	38.9 ± 7.0	11.3 ± 1.4
α−/− 8	4.90 ± 1.91	43.4 ± 12.0	45.5 ± 13.6	9.1 ± 7.6	43.5 ± 11.3	21.9 ± 5.8

IEL isolated from wt, β^−/− and α^−/− mice were incubated first with anti-Thy-1.2 mAb (biotinylated) and then with streptavidin–phycoerythrin and anti-TCR-γδ mAb (FITC-conjugated). These IEL were also incubated first with anti-Vγ1, anti-Vγ4, anti-Vγ7, or anti-Vδ4 mAb. After washing, the IEL were incubated with biotinylated goat anti-hamster IgG and subsequently counterstained with streptavidin-phycoerythrin and anti-TCR-γδ mAb (FITC-conjugated). The proportion of Thy-1⁺ cells in α^−/− γδ-IEL was significantly higher than those in wt (P < 0.05) and β^−/− (P < 0.01) γδ-IEL. The proportion of Vδ4⁺ cells in α^−/− γδ-IEL was significantly higher than those in wt (P < 0.01) and β^−/− (P < 0.01) γδ-IEL.