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The in vitro interaction of TEL and UBC9 requires the HLH domain. In vitro-translated (ivt) TEL (lane 1) or the deletion mutant TELΔHLH (lane 4) were incubated with GST-UBC9, washed, and separated by SDS/PAGE as described in Materials and Methods. Only the w. t. TEL (lane 3), but not the HLH deletion mutant (lane 6), was retained by UBC9.
