Figure 4.

TEL and UBC9 interact in mammalian cells. NIH 3T3 cells were transiently transfected with pCMV-Gal4-UBC9 and pVP16-TEL separately or together as indicated. The luciferase activity was normalized for transfection efficiency and plotted as shown. Bar 1, cells transfected with only the reporter plasmid. Addition of UBC9 alone (bar 2) or TEL alone (bar 3) does not result in significant change. However cotransfection of both plasmids (bar 4) results in about 8-fold increase of luciferase expression.