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. 1999 Jun 22;96(13):7479–7483. doi: 10.1073/pnas.96.13.7479

Figure 2.

Figure 2

Southern blot analysis to determine the transcriptional activity of the Piga alleles. (A) Southern blot analysis of DNA obtained from wt animals and mice hemi-, hetero-, or homozygous for the lox-Piga-lacZ gene. (B) Southern blot analysis of DNA isolated from different organs of a female mouse with a lox-ΔPiga-lacZ gene in almost all cells (mouse E, Fig. 3B). DNA was digested with PstI alone (P) or with PstI and SacII (PS). Southern blots were hybridized with the Sac-Bam DNA probe. The 10.4-kb fragment corresponds to lox-Piga-lacZ, the 8.1-kb fragment to lox-ΔPiga-lacZ, and the 7.0-kb fragment to the wt Piga gene. Digestion of the nonmethylated portion with SacII shortens each restriction fragment by 0.5 kb. Sp, spleen; Th, thymus; Li, liver; K, kidney; L, lung; B, brain. F (father) displays DNA of a male lox-Piga-lacZ(+) mouse, M (mother) shows DNA of a female EIIa-cre(+/+) mouse.