Figure 3.

Diagram summarizing methylation at the 5′ SacII restriction site in different tissues. (A) Methylation of wt and lox-Piga-lacZ in 3 mice heterozygous for lox-Piga-lacZ. (B) Methylation of wt and lox-ΔPiga-lacZ in 3 mice that had high levels of Piga gene recombination. The Southern blot analysis of mouse E is shown in Fig. 2B. DNA samples from the heart of mouse A and E and from the liver of mouse A and C were degraded. DNA from heart, lung, kidney, and spleen of mouse D were lost accidentally.