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. 1999 Jun 22;96(13):7490–7495. doi: 10.1073/pnas.96.13.7490

Figure 5.

Figure 5

Silibinin modulates protein levels of cyclin D1, CDKs, and CDKIs and increases binding of CDKIs to CDK2 in LNCaP cells. Dose- and time-dependent effect of silibinin on levels of cyclin D1 (A); CDK4 (B); CDK6 (C); Cip1/p21 (D); and Kip1/p27 (E). Cells were treated with silibinin for 20 hr or for the indicated time at 75 μg/ml; C, control cells treated with ethanol for 48 hr. Cell lysates were prepared and subjected to SDS/PAGE, Western blotting, and enhanced chemiluminescence detection as described in Materials and Methods. Shown also is the effect of silibinin on binding of CDKs with Cip1/p21 (F) and Kip1/p27 (G). Cells were treated with vehicle or 75 μg/ml of silibinin for 16 hr, and cell lysates were prepared. CDKIs binding with CDKs was determined as described in Materials and Methods. The data shown are representative of three independent experiments with similar findings.