Table 8.

Properties of PCR amplicons used for microarray resequencing of chimpanzee and gorilla: size, required mass and observed concentration, and volume added to pool

Chimpanzee PCR Amplicons	Size (bp)	Mass (ng)	Conc. (ng/mL)	Volume (uL)
h01F, h10R	6736	244.5	19.8	12.35
h09F, h13R	3582	130.0	11.1	11.71
h14F, h17R	2885	104.7	5	20.95
h18F, h18R	866	31.4	9.4	3.34
h19F, h19R	976	35.4	6.9	5.13
h20F, h22R	2679	97.2	12.5	7.78
h23F, h23R	807	29.3	11.1	2.64
Gorilla PCR Amplicons	Size (bp)	Mass (ng)	Conc. (ng/mL)	Volume (uL)
h01F, h06R	4082	148.2	19.4	7.64
h06F, h09R	2888	104.8	15.1	6.94
h10F, h10R	886	32.2	14.1	2.28
Gg11F, Gg12R	1420	51.5	14.7	3.51
h13F, h13R	815	29.6	20.6	1.44
h14F, h17R	2885	104.7	22.8	4.59
Gg18F, Gg18R	590	21.4	25.4	0.84