Figure 5.

Histology and microstructure of 3-weeks-old engineered cardiovascular constructs. The straining direction is from left to right. (a–c) H&E staining of engineered cardiovascular constructs cultured at 0, 4, and 8% strain, respectively. (d–f) Multiphoton images of cells (blue) and collagen (green) visualized at the surface of the engineered constructs, approximately 3 μm into the tissue. (g–i) Multiphoton images of cells and collagen visualized approximately 25 μm into the engineered tissue constructs