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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Nov 26;366(1):23–28. doi: 10.1016/j.bbrc.2007.11.056

Fig. 1. mtNOS activity and mitochondrial respiratory chain complexes.

Fig. 1

mtNOS activity in isolated liver (A, B, C) and brain (D, E, F) mitochondria were measured using oxyhemoglobin (A, D; expressed), citrulline radioassay (B, E), and chemiluminescence assay (C, F). Measurements were performed under the conditions that mitochondrial respiratory chain activity was not altered by exogenous respiratory substrates (no RS), RCC-I was activated (RCC-I), RCC-I was inactivated (Rot), or RCC-I was inactivated and mitochondria were energized from RCC-II (RCC-II). Where indicated mtNOS was inhibited by L-NMMA. Results are expresses as nmol NO per mg mitochondrial protein minute (A, D); nmol citrulline per mg mitochondrial protein (B, E), and pmol NOx per mg mitochondrial protein (C, F). *P < 0.05 RCC-I vs. no RS, Rot, RCC-II; $P < 0.05 vs. LNMMA.