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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Nov 26;366(1):23–28. doi: 10.1016/j.bbrc.2007.11.056

Fig. 4. mtNOS activity of submitochondrial particles.

Fig. 4

A) mtNOS activity was measured in submitochondrial particles by using citrulline assay and expressed as percentage of when submitochondrial particles were not supplied with exogenous respiratory substrates (No RS). Bars represent when RCC-I was activated by NADH in the absence (NADH) or presence of L-NMMA (NADH + LNMMA) or rotenone (NADH + Rot), when RCC-I was inactivated by rotenone and RCC-II was activated by succinate (Succ), or NADPH (NADPH) was present in place of NADH. B) mtNOS activity was measured by using NO-sensitive electrode under the conditions that submitochondrial particles were not supported with respiratory substrates (No RS), or RCC-I was activated by NADH in the absence (NADH) or presence of L-NMMA (NADH + LNMMA) or rotenone (NADH + Rot), or NADH was replaced with NADPH (NADPH). *P < 0.05 NADH vs. no RS, NADH + Rot, Succ, NADPH; $P < 0.05 vs. LNMMA.