. Author manuscript; available in PMC: 2008 Jul 1.

Published in final edited form as: Nat Struct Mol Biol. 2007 Dec 16;15(1):109–111. doi: 10.1038/nsmb1326

Table 1.

Effect of mutations on JMJD2A binding to H3K4me3 and H4K20me3 peptides

	K_d (μM)
Hybrid tudor domains of JMJD2A	H3K4me3 peptide	H4K20me3 peptide
Wild type	0.50 ± 0.03	0.40 ± 0.03
D939R	3.39 ± 0.14	85.87 ± 4.24
D945R	99.01 ± 5.84	0.77 ± 0.04
N940R	23.15 ± 1.45	1.00 ± 0.06
Y942A	1.52 ± 0.04	0.50 ± 0.03
Y942R	1.87 ± 0.08	0.76 ± 0.10
T968A	0.94 ± 0.05	0.21 ± 0.07
T968R	4.67 ± 0.10	0.70 ± 0.05

	H4K20me3 H18G peptide	H4K20me3 R19G peptide

Wild type	2.10 ± 0.11	21.93 ± 0.91

The K_d values were derived using ITC by fitting a one-site binding model and are reported with the associated s.d. determined by nonlinear least-squares analysis.