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. 1999 Jun 22;96(13):7496–7501. doi: 10.1073/pnas.96.13.7496

Figure 3.

Figure 3

The effect of gp120 on the CCR5–CD4 and CXCR4–CD4 association. (A) 3T3.CD4.CXCR4 cells were incubated with the anti-CXCR4 mAb 4G10 in the absence (−) or presence (+) (5 μg/ml) of HIV-1 IIIB gp120. The CD4 and CXCR4 were detected by using Western blotting with either the polyclonal anti-CD4 Ab T4–4 or 4G10. (B) 3T3.CD4.CCR5 cells were incubated with the anti-CCR5 mAb 5C7 in the absence or presence (5 μg/ml) of HIV-1 89.6 gp120. The CD4 and CCR5 were detected by using Western blotting as described in Fig. 1A. (C) The same as in B but gp120 from the R5 HIV-1 JRFL was used instead of the dual tropic 89.6.