Figure 5.

Colocalization of CD4 and CXCR4 (Left) or CCR5 (Right). A CXCR4 (or CCR5)-myc tag-expressing HeLa cell, double stained for CD4 (green) and CXCR4 (or CCR5) (red) (Upper). Colocalization of the two molecules is demonstrated by the yellow (red-green colocalization) staining, suggesting their clusterization. Correlation maps of these images (Bottom) show the regions of true overlap of green and red staining selected from the noncolocalized staining and the background fluorescence, represented as white dots.