Table 3. Clone Production and Sequencing Summary.
Type | Phase1 (1st isolate) | Phase2 (2nd isolate) | Total | |
ORF Target | CLOSED | 3557 | 277 | 3557 |
FUSION | 3557 | 327 | 3557 | |
Avg ORF size and range (bp) | ALL | 1268 (99–4,785) | 1491 (171–4,395) | 1268 (99–4,785) |
Clones for sequence validation | CLOSED | 3535 | 277 | 3812 |
FUSION | 3496 | 327 | 3823 | |
Number of reads | ALL | 20672 | 1957 | 22629 |
Average number of reads per clone | ALL | 2.9 | 3.2 | 2.9 |
Accepted clones | CLOSED | 3240 (91%) | 223 (81%) | 3463 (91%) |
FUSION | 3242 (92%) | 258 (79%) | 3500 (92%) | |
ALL | 6482 | 481 | 6963 | |
Accepted clone match perfect with reference | ALL | 6226 | 443 | 6669 (95.8%) |
Accepted clone with silent mutation(s) only | ALL | 94 | 13 | 107 (1.5%) |
Accepted clone with 1 mis-sense | ALL | 162 | 25 | 187 (2.7%) |
Rejected clones | ALL | 646 (9%) | 119 (20%) | 765 (10%) |
Clones rejected for linker changes | ALL | 158 | 43 | 201 (26%) |
Clones rejected for cds changes (ins/del/nonsense/mis) | ALL | 187 | 48 | 235 (31%) |
Clones rejected for no/incomplete/wrong assembly | ALL | 301 | 28 | 329 (43%) |
Accepted ORFs | CLOSED | 3097 (88%) | 222 (80%) | 3295 (93%) |
FUSION | 3075 (88%) | 256 (78%) | 3259 (92%) | |
ALL | 3387 | 453 | 3447 (97%) |