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. 2008 Jan 30;3(1):e1528. doi: 10.1371/journal.pone.0001528

Table 3. Clone Production and Sequencing Summary.

Type Phase1 (1st isolate) Phase2 (2nd isolate) Total
ORF Target CLOSED 3557 277 3557
FUSION 3557 327 3557
Avg ORF size and range (bp) ALL 1268 (99–4,785) 1491 (171–4,395) 1268 (99–4,785)
Clones for sequence validation CLOSED 3535 277 3812
FUSION 3496 327 3823
Number of reads ALL 20672 1957 22629
Average number of reads per clone ALL 2.9 3.2 2.9
Accepted clones CLOSED 3240 (91%) 223 (81%) 3463 (91%)
FUSION 3242 (92%) 258 (79%) 3500 (92%)
ALL 6482 481 6963
Accepted clone match perfect with reference ALL 6226 443 6669 (95.8%)
Accepted clone with silent mutation(s) only ALL 94 13 107 (1.5%)
Accepted clone with 1 mis-sense ALL 162 25 187 (2.7%)
Rejected clones ALL 646 (9%) 119 (20%) 765 (10%)
Clones rejected for linker changes ALL 158 43 201 (26%)
Clones rejected for cds changes (ins/del/nonsense/mis) ALL 187 48 235 (31%)
Clones rejected for no/incomplete/wrong assembly ALL 301 28 329 (43%)
Accepted ORFs CLOSED 3097 (88%) 222 (80%) 3295 (93%)
FUSION 3075 (88%) 256 (78%) 3259 (92%)
ALL 3387 453 3447 (97%)