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. Author manuscript; available in PMC: 2008 Nov 16.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Sep 10;363(2):381–387. doi: 10.1016/j.bbrc.2007.08.181

Fig. 3.

Fig. 3

UV-cross-linked RNA is purified from p53-null cells by anti-p53 antibodies. UV-cross-linked MCF-7 (TP53 +/+) cell lysate (M) or PC-3 (tp53 −/−) cell lysate (P) treated with RNase A and cross-linked RNA fragments were labeled before IP. (A) Western detection of p53. (B) Membrane from (A) exposed to film to visualize labeled RNA. (C) CLIP with anti-p53 antibodies. MCF-7 cell lysate treated with RNase A and subjected to IP with the indicated antibodies and Western detection of p53. (D) Radiolabeled RNA IP from p53-null PC-3 cells.