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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Dev Biol. 2007 Oct 24;313(1):307–319. doi: 10.1016/j.ydbio.2007.10.023

Fig. 4. A 1.55 kb Xlsox3 upstream regulatory region is partially conserved in X. tropicalis and mimics endogenous sox3 expression.

Fig. 4

(A–F) WISH of transgenic embryos expressing the Xlsox3 –GFP reporter construct. (A) Vegetal view of a stage 10.5 and (B) stage 12 embryo with dorsal to the top. (C) Anterior view of a stage 21 embryo on top with a dorsal view of the same embryo directly below. (D) Lateral view of a stage 24 embryo with a dorsal view of the same embryo directly below. (E) Dorsal view of a stage 27 embryo. (F) Lateral view of the head of a stage 33 embryo. The line represents the sox3 upstream regulatory region and −1.55 represents the distance in kb from the ATG. The green box represents the EGFP coding region and the black box the SV40 polyA region. (G) Evolutionary conserved regions between X. laevis and X. tropicalis sox3. A diagram of the sox3 regulatory region used in (A–F) is at the top with a percent identity plot (pip) of Xlsox3 aligned to a homologous region of Xtsox3 below it. At the bottom is a pip of Xtsox3 aligned to Xlsox3. The blue box represents a 484 bp region in X. laevis which does not align to the Xtsox3 sequence. The gray box represents a 30 bp region which is conserved between mouse and Xlsox3. The X-axis of the pip is the length of the sequence in bases or kilobases as designated. The Y-axis spans 50–100% nucleotide identify with the light horizontal line in the center representing 75% nucleotide identity. The red regions under the curve represent evolutionary conserved blocks. Two large blocks of the Xlsox3 upstream regulatory region align with Xtsox3. In Xtsox3, the first block (farthest from the ATG) is split into two blocks.