Skip to main content
. Author manuscript; available in PMC: 2008 Dec 22.
Published in final edited form as: FEBS Lett. 2007 Dec 3;581(30):5904–5910. doi: 10.1016/j.febslet.2007.11.072

Fig 1. CITED2 knockdown induced morphological changes in RKO cells.

Fig 1

(A) RKO cells were transduced with plko1 empty lentiviral vector or plko1-shCITED2, and selected with puromycin for 3 weeks. The pools of puromycin-resistent cells were used for total RNA isolation and RT-PCR analyses. (B) Whole cell extracts were prepared and mouse anti-CITED2 (Novus Biologicals) and anti-GAPDH (Chemicon) antibodies were used for immunoblotting (IB). (C) Phase contrast microscopy of live cells (magnification: 200×). (D) Cells were stained with Alexa fluor 488 phalloidin (Molecular Probes) and examined with confocal microscope (magnification: 400×).