Skip to main content
. Author manuscript; available in PMC: 2008 Dec 22.
Published in final edited form as: FEBS Lett. 2007 Dec 3;581(30):5904–5910. doi: 10.1016/j.febslet.2007.11.072

Fig 3. CITED2 knockdown does not affect the β-catenin pathway.

Fig 3

(A) Whole cell extract (WCE), cytoplasmic (Cyto) and nuclear (Nucl) fractions of RKO cells were prepared for immunoblotting. Rabbit anti-HDAC1 (Santa Cruz Biotechnology) and mouse anti-β-catenin (BD Biosciences) were used for immunoblotting. HDAC1 was used as a marker for the nuclear fraction, and GAPDH was used as a marker for the cytoplasmic fraction. (B) Total RNA was isolated from cells for RT-PCR as described above.