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. Author manuscript; available in PMC: 2008 Dec 22.
Published in final edited form as: FEBS Lett. 2007 Dec 3;581(30):5904–5910. doi: 10.1016/j.febslet.2007.11.072

Fig 4. CITED2 knockdown increased MMP-13 expression and enzyme activity in RKO cells.

Fig 4

(A) RKO cells were transfected with a control siRNA (Ambion) or CITED2-specific siRNA. Twenty hours after transfection, total RNA was isolated for RT-PCR. (B) Cells were seeded in 12-well plates. Twelve hours later, cells were washed and incubated in serum-free medium for another 24 h. The medium was collected, centrifuged; and the supernatant was used to assay MMP-13 activity.