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. Author manuscript; available in PMC: 2008 Dec 22.
Published in final edited form as: FEBS Lett. 2007 Dec 3;581(30):5904–5910. doi: 10.1016/j.febslet.2007.11.072

Fig 5. CITED2 knockdown increased MMP-13 expression and cell invasiveness in SW480 cells.

Fig 5

(A) SW480 cells were transfected with a control siRNA (Ambion) or CITED2-specific siRNA. Twenty hours after transfection, total RNA was isolated for RT-PCR. (B) SW480 cells were transduced with plko1 empty lentiviral vector or plko1-shCITED2, and selected with puromycin for 3 weeks. The pools of puromycin-resistent cells were used for RT-PCR (upper panels) and Western blot (lower panels) analyses. (C) Matrigel invasion assays of cells in (B) were performed as described in Fig 2B. Twenty-four hours after invasion, cells on the lower surface of the membrane were stained with H&E and counted. Data shown are means ± SEM of cells from three independent experiments, and ten fields were counted from each experiment.