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. Author manuscript; available in PMC: 2008 Dec 22.
Published in final edited form as: FEBS Lett. 2007 Dec 3;581(30):5904–5910. doi: 10.1016/j.febslet.2007.11.072

Fig 6. Butyrate upregulated CITED2 expression and downregulated MMP-13 expression in RKO cells.

Fig 6

(A) RKO cells were treated with 5 mM sodium butyrate for 16 h. Total RNA was isolated for RT-PCR. (B) RKO cells transfected with pMK17 reporter, then treated with 2.5 mM sodium butyrate for 20 h prior to luciferase reporter assays that were normalized to protein. Results are presented as means ± SEM of three experiments. (C) RKO and SW480 cells were transfected with pCMV10 vector (Vec) or pCMV10/HA-CITED2 (HA-C2). Two days later, cells were collected and total RNA was isolated for RT-PCR analyses.