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. 2007 Dec 9;2:23. doi: 10.1186/1750-1326-2-23

Figure 1.

Figure 1

Munc13-1 increases constitutive and phorbol-stimulated sAPPα secretion in a fashion that is independent of the integrity of its phorbol-sensing C1 domain. Levels of soluble APPα (sAPPα) ectodomain were measured by Western blotting of cell supernatants with anti-APP antibody, 6E10, following the treatment of cells with DMSO (-) or 100 nM PMA (+) for 2 hours in a 37°C, 5% CO2 cell culture incubator. Levels of holoAPP were measured from cell lysates with anti-APP antibody 369. Levels of Munc13-1 wild type and Munc13-1 H567K mutant proteins were measured by anti-GFP antibody. Equal protein loading was verified by measuring the levels of actin protein in all cell lysates.