Figure 6.

Co-localisation of DAZL and VASA in 2nd trimester ovary and testes. In the 2nd trimester (ovary, a, 17 wk; c, 14 wk; testes b, 15 wk; d, 16 wk) three populations of germ cells were identified based on their patterns of expression of DAZL (red) and VASA (green). 1stly a rare population of cells which had nuclear DAZL (arrows), 2ndly cells which co-expressed both proteins (population 2) and thirdly the most prevalent group which were VASA positive/DAZL negative (population 3). Although few cells in population 2 were detected in testes these germ cells were found as distinct groups within the ovary (see panel c).