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Two-dimensional SDS/PAGE analysis of SodCII (ASP71) in wild-type Salmonella and a sodCII mutant. Wild-type UK1 (A and B) and sodCII mutant JF3912 (C and D) were grown to logarithmic phase at pH 7.7 in minimal EG medium. Cells were either processed directly for two-dimensional SDS/PAGE (A and C) or acid shocked for 1 hr at pH 4.4 before processing (B and D). The arrow indicates the position of SodCII. The panels show equivalent small areas of larger gels.
