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. Author manuscript; available in PMC: 2008 Nov 15.
Published in final edited form as: Mol Cell Endocrinol. 2007 Aug 19;278(1-2):29–35. doi: 10.1016/j.mce.2007.08.004

Figure 2.

Figure 2

Luciferase activity of granulosa cells cotransfected with a −191/+9 IGFBP-3 promoter construct and a TAFb expression vector. Cells were cultured in 10% FBS for 18h and transfected with either a −191/+9 IGFBP-3 promoter construct of a combination of the IGFBP-3 construct and a TAF4b expression vector in PFLAG, using the Lipofectin method. Cells were maintained in 10% FBS until 95% confluent. They were then incubated in serum-free medium overnight before being exposed to 100 ng/ml FSH for 3h. Control cells were treated with an equal volume of PBS. Beta galactosidase was used as an internal control. All transfections were done in triplicate and repeated 3 times with different batches of granulose cells. Results are mean±SEM.