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. Author manuscript; available in PMC: 2008 Nov 15.
Published in final edited form as: Mol Cell Endocrinol. 2007 Aug 19;278(1-2):29–35. doi: 10.1016/j.mce.2007.08.004

Figure 3.

Figure 3

Expression of TAF4b mRNA by granulosa cells following FSH treatment. Granulosa cells were cultured to 95% confluence, serum starved overnight and treated with 100 ng/ml FSH for 0, 30, 60, 180 or 360 minutes. Total RNA was extracted and subjected to RT-PCR. Primers for the housekeeping gene GAPDH were used as internal controls. Densitometry was used to quantify the TAF4b mRNA levels relative to the GAPDH mRNA at each time point. The blot is representative of 3 different blots, with different batches of granulose cells.