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. 2008 Jan 22;6(1):e7. doi: 10.1371/journal.pbio.0060007

Figure 5. Altered Expression of PDLP1 Changes Plasmodesmal Trafficking.

Figure 5

PDLP1 KO lines (A) or PDLP1a:HA overexpressing lines (B) were assessed for plasmodesmal trafficking potential by counting the number of GFP recipient cells surrounding primary bombardment sites. From three double KO lines, only At2g33330×At1g04520 and At5g43980×At2g33330 showed altered GFP diffusion. (Data for At2g33330×At1g04520 were obtained from two independent experiments and 96 bombardment sites each for wild-type (WT) and KO lines (p ≤ 0.05); data for At5g43980×At2g33330 were obtained from three independent experiments and 57 and 93 bombardment sites for WT and KO lines, respectively (p ≤ 0.01). (B) Transgenic Arabidopsis plants expressing 35S::PDLP1a:HA (or 35S::PDLP1a:GFP; unpublished data) exhibited a dwarf phenotype (right, upper picture) when compared with nontransformed (NT) plants. This dwarf phenotype showed a positive correlation with transgene copy number and protein accumulation, assessed by western analysis with anti-HA antibody (right, lower picture). Bombardment of the less-dwarfed hemizygous plants (Hemi; left) showed a dramatic reduction in GFP diffusion (data collected from two independent experiments and 70 and 114 bombardment sites for the NT and hemizygous plants, respectively; p ≤ 0.0001). Homo, homozygous.

Bars indicate the standard error of the mean.