Table 2.
Behavioral phenotyping of the V73KO mice. Number of subjects is in brackets.
| Age | Sex | +/+ | +/− | −/− | P | |
|---|---|---|---|---|---|---|
| Observational Batery | ||||||
| Piloerection, bald Patches | 8 w | M, F | No (7) | No (11) | No (6) | |
| Ear/foot color | 8 w | M, F | pink | pink | pink | |
| Posture, gait, grooming, righting | 8 w | M, F | normal | normal | normal | |
| Reflexes (Eye blink, ear twitch, wisker orienting) | 8 w | M, F | normal | normal | normal | |
| Abnormal behavior (cicrling, stereotypy, freezing) | 8 w | M, F | No | No | No | |
| Weight | 8 w | M | 35 g (10) | 38 g (10) | 31 g (6) | 0.25 |
| Locomotion (60min, total distance traveled in m) | ||||||
| Saline | 2−3 M | M, F | 2.58 (10) | 1.88 (7) | 3.44 (12) | 0.31 |
| Cocaine (10 mg/kg, s.c.) | 2−3 M | M, F | 3.84 (10) | 6.81 (7) | 5.50 (12) | 0.45 |
| Ethanol (2 mg/kg, i.p.) | 2−3 M | M, F | 6.43 (10) | 7.05 (10) | 9.18 (10) | 0.52 |
| Morphine (3 mg/kg, s.c.) | 2−3 M | M, F | 2.46 (10) | 2.77 (10) | 2.28 (10) | 0.95 |
| Conditioned Place Preference (Cocaine, 10 mg/kg, s.c.) | ||||||
| Time change in drug-paired environment (s) | 2−3 M | M, F | 234 (9) | 193 (10) | 0.70 | |
| Motor functions | ||||||
| latency to fall off of an accelerating rotarod (s) | 8 w | M, F | 253 (10) | 206 (13) | 252 (12) | 0.19 |
| 6 M | M | 203 (14) | 192 (13) | 228 (11) | 0.47 | |
| 6 M | F | 233 (8) | 265 (11) | 281 (10) | 0.15 | |
| 12 M | M | 159 (10) | 132 (6) | 172 (7) | 0.59 | |
| 12 M | F | 188 (17) | 219 (20) | 258 (14) | 0.07 | |
| Learning in Morris Water Maze | ||||||
| Latency to platform quadrant (s) | 2−3 M | M, F | 2.3 (10) | 2.9 (10) | 1.9 (13) | 0.32 |
| Latency to new location (s) | 2−3 M | M, F | 1.7 (10) | 2.8 (10) | 2.9 (13) | 0.35 |
| Time in new/time in old | 2−3 M | M, F | 2.4 (10) | 2.2 (10) | 2.2 (13) | 0.92 |
| Breeding behavior | ||||||
| Estrous cycle -cycle duration (days) | 2−3 M | F | 6.6 (12) | 8.6 (12) | 0.25 | |
| Latency to produce a litter (days) | 2−3 M | F | 32 (13) | 33 (10) | 0.87 | |
| Litter size (# of pups) | 2−3 M | F | 6.9 (13) | 6.2 (10) | 0.61 | |
| Latency to collect 4 days old pups (s) | 2−3 M | F | 23 (6) | 31 (5) | 0.66 | |
| Olfaction | ||||||
| Latency to approach opposite sex's urine (s) | 2−3 M | M | 60 (12) | 43 (5) | 41 (10) | 0.64 |
| 2−3 M | F | 15 (10) | 52 (12) | 101 (11) | 0.0007* | |
| Latency to approach peanut butter odor (s) | 2−3 M | M | 33 (15) | 36 (13) | 33 (11) | 0.41 |
| 2−3 M | F | 53 (12) | 97 (18) | 92 (13) | 0.13 | |
| Olfactory discrimination (%correct) | ||||||
| 100 % odorant A | 2−3 M | M, F | 82 (11) | 92 (12) | ||
| 60 % odorant A, 40 % odorant B | 2−3 M | M, F | 79 (11) | 72 (12) | ||
| 58 % odorant A, 42 % odorant B | 2−3 M | M, F | 67 (11) | 69 (12) | ||
| Avoidance of aversive stimulus (TMT) | ||||||
| Time spent in TMT environment (s) | 2−3 M | M, F | 259 (11) | 338 (19) | 0.045* | |
| Analgesia | ||||||
| Latency to tail flick (53 °C water bath) | 2−3 M | M, F | 5.9 s (10) | 5.6 s (11) | 5.8 s (11) | 0.90 |
| Latency to jump/lick a paw (55 °C hot plate) | 2−3 M | M, F | 10.9 s (10) | 15.3 s (11) | 14.7 s (11) | 0.55 |
| Seizures: latency to tonic/clonic (s) | ||||||
| Pentylene tetrazole 80 mg/kg | 2−3 M | M, F | 111 (4) | 96 (4) | 0.39 | |
| Pentylene tetrazole 60 mg/kg | 2−3 M | M, F | 181 (14) | 289 (14) | 0.39 | |
| Anxiety | ||||||
| Elevated + maze — Latency to open (s) | 2−3 M | M, F | 34 (10) | 69 (10) | 48 (10) | 0.65 |
| Time in open (s) | 2−3 M | M, F | 37.4 (10) | 26.7 (10) | 30.7 (10) | 0.54 |
| Entries to open | 2−3 M | M, F | 5.5 (10) | 5.3 (10) | 4.9 (10) | 0.90 |
| Dark box - Latency to emerge (s) | 2−3 M | M, F | 40 (12) | 33 (7) | 39 (12) | 0.86 |
| Time in light compartment (s) | 2−3 M | M, F | 241 (12) | 196 (7) | 139 (12) | 0.28 |
| Open field - Time in center (s) | 2−3 M | M, F | 84 (12) | 40 (7) | 92 (12) | 0.17 |
| Number of entries | 2−3 M | M, F | 129 (12) | 104 (12) | 0.52 | |
| Novel object exploration (s) | 2−3 M | M, F | 26 (11) | 22 (9) | 25 (10) | 0.9 |
| Sress-Induced Anxiety | ||||||
| Dark box - Latency to emerge (s) baseline | 2−3 M | M, F | 481 (12) | 472 (12) | 0.96 | |
| stress | 2−3 M | M, F | 140 (12) | 2178 (12) | 0.025 | |
| - Time outside (s) baseline | 2−3 M | M, F | 241 (12) | 193 (12) | 0.16 | |
| stress | 2−3 M | M, F | 240 (12) | 136 (12) | 0.057 | |
| Open field - Time in center (s) baseline | 2−3 M | M, F | 83 (12) | 92 (12) | 0.76 | |
| stress | 2−3 M | M, F | 50 (12) | 24 (12) | 0.07 | |
| Number of entries baseline | 2−3 M | M, F | 129 (12) | 104 (12) | 0.49 | |
| stress | 2−3 M | M, F | 63 (12) | 32 (12) | 0.11 |
*
Not replicated in the subsequent experiments