Total number of cells per gram of seabream intestine tissue as counted under a Neubauer chamber after different isolation protocols and their viability (assessed by PI staining). Results are expressed as mean ± sd (n= 5 fish). One-way analysis of variance and Tukey test were conducted when necessary. Different letters stand for statistically significant differences (p<0.05) between isolation protocols.
Treatment	Numbers of cells/g tissue x 107	Viability (%)
DTT 10 min	0.26 ± 0.14a	65 ± 9.6a
Collagenase 0 mg/ml
30 min	8.15 ± 3.4c	82.5 ± 6.3b
60 min	42.8 ± 37.9e	83.5 ± 9.1b
120 min	48.2 ± 6.7e	76.6 ± 6.5b
Collagenase 0.15 mg/ml
30 min	19.4 ± 0.9d	82.6 ± 4.7b
60 min	22.6 ± 2.4d	80.5 ± 4.5b
120 min	40.5 ± 3e	74 ± 4.6b
Collagenase 0.37 mg/ml
30 min	18.7 ± 7.6d	88 ± 7.1b
60 min	48.75 ± 3e	82.75 ± 7.1b
120 min	75.6 ± 5.1f	77 ± 2.8b
Mechanical stripping	4.95 ± 1.5b	82.2 ± 2b