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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Dec 3;366(1):156–161. doi: 10.1016/j.bbrc.2007.11.116

Figure 3.

Figure 3

LOX-PP directly inhibits MEK2 activity, and not Erk2. Recombinant active MEK2 ( 1 ng) was incubated with 6.7 μg of inactive Erk2 in the presence or absence of recombinant LOX-PP purified from a bacterial expression system [LOX-PP (b)] [9] or from a mammalian expression system [LOX-PP (m)] at 30o C for 15 min. This incubation is stage I (Stg. I). Aliquots of Stage I reactions were then assayed for the activity of Erk2 by measuring the phosphorylation of myelin basic protein in the presence of [γ32P]-ATP [Stage II reaction (Stg. II)]. Some reactions contained LOX-PP only in the Stage II reaction. Data are means +/− SD of triplicate assays, and are from a representative experiment done three times with similar results (*, p<0.05).