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. 2007 Nov;16(11):2531–2541. doi: 10.1110/ps.073007207

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Figure 3. — Purification of his-tagged Cd36 from High Five membranes by Ni-NTA affinity chromatography. Purification fractions for Cd36–6His (A) and Cd36–12His (B) were subjected to 10% SDS-PAGE and proteins visualized by colloidal blue staining. (Lane 1) Standards, (lane 2) membrane preparation, (lane 3) OG-solubilized material, (lane 4) flow-through, (lanes 5–8) imidazole washes, pH 8 (20–35 mM for Cd36–6His, 60–120 mM for Cd36–12His), (lane 9) wash at pH 6.8, (lanes 10–13) elutions at pH 6.8 with 120 mM imidazole (Cd36–6His) or 250 mM imidazole (Cd36–12His).