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. 2007 Nov;16(11):2350–2359. doi: 10.1110/ps.073119507

Figure 3.

Figure 3.

Determination of the amount of E. coli used for screening. (A) The Sg13009 strain of E. coli was induced and the lysate analyzed by SDS-PAGE along with known amounts of purified H152C (34 kDa). (B) PhosphorImager was used to quantify the H152C bands and the data were graphed. Arrows are drawn from the 20-pmol amount of H152C (1/100 of 2 nmol) to intersect the linear transformation and then to the corresponding number of counts on the Y-axis. (C) The amount of E. coli needed to produce 2 nmol of H152C was estimated. The counts produced by the purified protein were used to determine the corresponding volume of E. coli culture (0.5 mL) needed to produce 2 nmol of H152C.