Figure 10.
Kinetic refolding of isolated domains of γSWT (inset shows completion of γSWT refolding kinetics reaction), γSN, and γSC. Protein was unfolded at high GuHCl concentration, then diluted into 100 mM sodium phosphate, 1 mM EDTA, 5 mM DTT (pH 7.0) buffer for a final protein concentration of 10 μg/mL. Protein tryptophan fluorescence emission at 350 nm was recorded every second, and data were normalized for comparison. All experiments were performed at 18°C. The final GuHCl concentration was 0.55 M for γSWT, γSN, and γSC (see text for details).