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Thermal denaturation of γDWT, γSWT, and their individual domains. Samples were prepared at 100 μg/mL protein concentration in 10 mM sodium phosphate buffer (pH 7.0). CD signal at 218 nm was monitored as the temperature was increased from 25°C to 90°C. Data were normalized and the native fraction was calculated (see Materials and Methods). (A) (♦) γDWT, (•) γDN, and (▪) γDC. (B) (▴) γSWT, (◢)γSN, and (◣) γSC.
