Figure 1.

Fate of murine class I MHC heavy chains in US11⁺ or US2⁺ cells. Control (nontransfected), US11⁺, or US2⁺ cells were infected with vaccinia virus expressing H-2K^b (lacking the cytoplasmic tail; A), K^d (B), D^b (C), D^d (D), or L^d (E), labeled with [³⁵S]methionine for 10 min and chased as indicated. Immunoprecipitated murine class I molecules were resolved on a 12.5% SDS–polyacrylamide gel and visualized by fluorography. The position of the breakdown intermediate is indicated with an asterisk.