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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Mol Biochem Parasitol. 2007 Oct 6;157(1):73–82. doi: 10.1016/j.molbiopara.2007.10.003

Fig. 4.

Fig. 4

Southern blot of genomic DNA confirms spontaneous LOH and CRE-mediated loss of LHTL cassette at the PYR6-5 locus. C and CTr denote cells transfected with pLEW100Cre stably or transiently, respectively. In the PYR6-5 locus, clones are wild type (+/+), heterozygous (+/H) or homozygous (H/H) for LHTL, prior to CRE induction. In lanes 6–9, cells were induced with doxycycline and selected in GCV. Blot was probed for HYG, which detects LHTL and a smaller band from a fragment of HYG, which is present in all SM-derived cells. The blot was reprobed for PYR6.