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. 2004 Jan 19;199(2):185–197. doi: 10.1084/jem.20031064

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Copyright © 2004, The Rockefeller University Press

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Figure 7. — Quantitative chemokine mRNA expression in the CNS. The relative amount of mRNA transcripts for (A) MCP-1 and (B) MIP-1α was analyzed from spinal cord tissue of MBP-EAE (MBP), S100β-EAE (S100), and control animals (control) by dot blot hybridization at days 2 and 4 after transfer. Note the massive up-regulation of MCP-1 and MIP-1α mRNA in the CNS of T_MBP cell–treated, but not in the T_S100β cell–treated, animals. Amplification of β-tubulin and analysis on ethidium bromide–stained agarose gels demonstrated intact RNA in all samples (not depicted). The data were confirmed in a second set of independently prepared samples.