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. 2004 Jan 19;199(2):255–264. doi: 10.1084/jem.20031519

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Copyright © 2004, The Rockefeller University Press

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Figure 8. — Abs eluted from nephritic kidneys of NZM2328 and NZM.C57Lc4 mice are reactive with proteins within the kidney and liver extracts. Proteins in the kidney (left) and liver (right) extracts were separated on a 12% SDS-PAGE, transferred to nitrocellulose paper, and used for analyzing the reactivity of Abs eluted from the nephritic kidneys of NZM2328 (lanes 2 and 3) and NZM.C57Lc4 (lanes 4 and 5) mice. Each lane is equivalent to 60 μg total protein. Abs were used at a concentration of 30 (lanes 2 and 4) and 10 μg/ml (lanes 3 and 5). Lane 1 in both panels represents reactivity of the goat anti–mouse IgG–horseradish peroxidase conjugate with the extracts. Numbers on the left represent molecular weights in kilodaltons.