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. 2004 Jun 7;199(11):1455–1465. doi: 10.1084/jem.20040139

Figure 5.

Figure 5.

Prevention of diabetes transfer by expanded Tregs. (A) Activated diabetogenic BDC2.5 CD4+ CD62L+ CD25− cells (3.5 × 105) were cotransferred with BDC2.5-expanded Tregs to 8-wk-old NOD. RAG−/− recipients at the indicated ratio. The blood glucose for individual recipient mouse was monitored and plotted to access diabetes. n = 3 for no Tregs and 1:9 groups; n = 4 for 1:1 and 1:3 groups. Results are representative of three independent experiments. (B) Diabetes was induced in 6-wk-old NOD.RAG−/− mice in the same manner as described in A, except that the number of transferred expanded Tregs from GAD286 TCR Tg mice and BDC2.5 TCR Tg mice equaled the number of transferred Teffs (n = 3 mice/group). (C) Diabetes was induced in 5–8-wk-old NOD.RAG−/− or NOD.TCR-α−/− recipients by injection of 25 × 106 pooled spleen and LN cells from diabetic donors (n = 8). Some recipient mice were coinjected with expanded Tregs from NOD (2 × 106, n = 3; 5 × 106, n = 3; 8 × 106, n = 4) or BDC2.5 TCR Tg mice (2 × 106, n = 4). Results represent two independent experiments.