Figure 3.

Unc119 specifically interacts with Lck and Fyn. (A) Unc119 specifically interacts with Lck in T cells. T cells were prepared as in Fig. 1 B, immunoprecipitated (IP) with indicated antibodies. Pellets and/or supernatants (see Fig. 1 B) were Western blotted with anti-Unc119 antibody and reprobed with anti-Lck. NS, nonspecific band. (B) Unc119 interacts with Fyn in T cells. T cells were prepared as in Fig. 1 B and immunoprecipitated (IP) with anti-Unc119. Pellets and/or supernatants (Fig. 1 B) were Western blotted with antibodies against indicated proteins and reprobed with anti-Unc119 (bottom). (C) GST-Unc119 interacts with Lck and Fyn. Cells lysates, prepared as aforementioned, were precipitated with GST or GST-Unc119. Pellets and/or supernatants were Western blotted with antibodies against indicated proteins.