Figure 1.

Micromanipulation of daughter cell pairs and granddaughter cell pairs derived from single CD34⁻KSL cells in vitro. (A) After single CD34⁻KSL cells underwent first divisions in the presence of SCF, SCF + IL-3, SCF + TPO, or SCF + IL-3 + TPO, members of daughter cell pairs were separated by micromanipulation and further cultured in the presence of SCF + IL-3 + TPO + EPO to permit full differentiation along myeloid lineage. (B) After single CD34⁻KSL cells underwent first divisions in the presence of SCF + IL-3 or SCF + TPO, members of daughter cell pairs were separated into wells containing SCF + IL-3 or SCF + TPO. After each daughter cell underwent second division, granddaughter cells were separated and individually cultured in the presence of SCF + IL-3 + TPO + EPO.