Table III.

Differentiation Potential of Paired Granddaughter Cells

	Differentiation potential
Cytokine	One	The other	No. of pairs
SCF + IL-3	nmEM	nmEM	15
	nmEM	nm	6
	nmEM	mEM	5
	nmEM	nmM	2
	nmEM	nmE	1
	nmEM	mM	1
	nmEM	M	1
	nmM	nmE	2
	nmM	nEM	1
	nm	nEM	2
SCF + TPO	nmEM	nmEM	21
	nmEM	nm	5
	nmEM	nmM	2
	nmEM	nmE	1
	nmEM	M	1
	nEM	m	1

After single CD34⁻KSL cells divided in the presence of the cytokines shown, the two daughter cells were separated. After individual daughter cells underwent division under the same condition, the granddaughter cells were again separated and were allowed to form colonies in the presence of SCF + IL-3 + TPO + EPO (Fig. 1 B). In total, 59 and 47 cell pairs generated in the presence of SCF + IL-3 and SCF + TPO were serially manipulated. Only the pairs derived from nmEM cells are presented. The probability of asymmetric division induced by SCF + IL-3 (0.58, 21/36) was significantly greater than that induced by SCF + TPO (0.32, 10/31; P = 0.0492).