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. 2004 Feb 2;199(3):315–321. doi: 10.1084/jem.20031142

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — V(D)J recombination on chromosomal substrates. (A) structure of the MX-RSS-EGFP/ires-HuCD4 construct before and after inversional V(D)J recombination. In the germline configuration, an inverted EGFP cassette is flanked by two RSS. Upon V(D)J recombination, the EGFP gene is reoriented and productively transcribed from the LTR promoter. (B) Wild-type OTEL- and Artemis-deficient GUETEL cells were transduced with MX-RSS-EGFP/ires-HuCD4 resulting in 43 and 80% CD4⁺ cells, respectively. Recombination of the substrates is initiated by transient transfection of RAG1/RAG2 expression constructs with or without Artemis. (C) Mean results of 6 V(D)J recombination experiments in GUETEL/RSS cells. The V(D)J recombination activity is calculated relative to the recombination frequency obtained with RAG1 (R1), RAG2 (R2), and wild-type Artemis (Arte wt).